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KMID : 1149220110190010183
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Korean Journal of Oriental Medical Prescription
2011 Volume.19 No. 1 p.183 ~ p.194
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Estimation of shelf-life by long-term storage test of Pyungwi-san
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Seo Chang-Seob
Kim Jung-Hoon
Lim Soon-Hee
Shin Hyeun-Kyoo
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Abstract
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Objectives:To estimate the shelf-life by long-term storage test of Pyungwi-san.
Methods:Experiments were conducted to evaluate the stability such as the selected physicochemical, heavy metal, microbilogical experiment under an acceleration test and long-term storage test of Pyungwi-san in different storage under room temperature, refrigeration and freezing. Futhermore, HPLC analysis was performed for the determinations of glycyrrhizin in the Pyungwi-san on an Inertsil ODS-3 column(250 mm 4.6 mm, 5 um) using solvent 35% acetonitrile include 0.05% phosphoric acid at 254 nm. The flow rate was 1.0 mL/min.
Results:The significant change was not showed in pH, heavy metal, microbiological, identification test and quantitative analysis based on acceleration test and long-term storage test. Retention time of glycyrrhizin in HPLC chromatogram was about 16.065 min and calibration curve showed good linearity(R² = 0.9999). The contents of glycyrrhizin in acceleration test and long-term storage test were 0.068~0.076 mg/mL and 0.066~0.077 mg/mL, respectively. Shelf-lifes of room temperature, refrigeration and freezing by long-term storage test were predicted 41, 24 and 34 months, respectively.
Conclusions :The suggested shelf-life would be helpful on the storage and distribution of herbal medicine.
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KEYWORD
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Shelf-life, Pyungwi-san, Glycyrrhizin, Herbal medicine
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